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Iranian Journal of Veterinary Clinical Sciences
Serological survey of Mycoplasma synoviae infection in industrial poultry of Ardabil province by using RSPA and ELISA


Aidin Azizpour    unknown



Mycoplasma synoviae (MS) is an important pathogens of poultry and turkeys, and causes respiratory infection, lameness, decreased growth rate, loss of egg production and hatch reduction. This study was conducted to determine the seroprevalence of MS infection in industrial poultry of Ardabil province, northwestern Iran, and to find the possible association of prevalence of infection with some potential factors. Between October 2012 and April 2013, 655 blood samples were collected from 36 industrial flocks. Serum samples were examined by Rapid Serum Plate Agglutination (RSPA) and ELISA tests. From 36 flocks, 13 flocks (36.1%) in RSPA and 11 flocks (30.6%) in ELISA were positive. From 655 serum samples, 261 samples (39.8%) and 207 samples (31.6%) were positive in RSA and ELISA tests, respectively. In RSPA, sensitivity, specificity and correlation rate of ELISA were 84.6%, 92.5% and -0.352, respectively. The highest rate of seroprevalance with statistical difference (p<0.001) was in winter. 31.8% of MS prevalence was detected in the female type and 28.9% in the male type. The capacity of the flocks was classified into four groups and the highest MS seroprevalance was seen in the flocks including more than 20000 birds. The prevalence of MS in Namin city was significantly (p<0.05) higher (32.2%) as compared to other regions. The results of this study showed that factors such as sex of birds, size of flocks, seasonal variations and density of flocks in sampling regions were effective in MS. Also, the seroprevalence of MS infection is relatively higher in the industrial flocks of Ardabil Province. It needs to respect the biosecurity and sanitation principles and providing free MS chickens.


Industrial poultry RSPA ELISA Mycoplasma synoviae  

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Journal of Materials Science: Materials in Electronics
Impact Factor
2000 - 2020
Open Access Articles